Biomolecules

How To Analyse Chemical Composition?

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1. We can continue asking in the same way, what type of organic compounds are found in living organisms? How does one go about finding the answer? To get an answer, one has to perform a ____.

2. We can take any living tissue (a vegetable or a piece of liver, etc.) and grind it in ___ using a mortar and a pestle. We obtain a thick slurry. If we were to strain this through a cheesecloth or cotton we would obtain two fractions. One is called the filtrate or more technically, the ___ pool, and the second, the retentate or the ___ fraction.

3. Scientists have found ____ in the acid-soluble pool.

4. To identify a particular organic compound from a living tissue, one must extract it, apply separation techniques, then ____ the compound before analysing its molecular formula and structure.
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In higher classes you will learn about how to analyse a living tissue sample and identify a particular organic compound. It will suffice to say here that one extracts the compounds, then subjects the extract to various separation techniques till one has separated a compound from all other compounds. In other words, one ____ a compound. Analytical techniques, when applied to the compound give us an idea of the molecular formula and the probable structure of the compound.

5. All the carbon compounds obtained from living tissues are called ____.

6. However, living organisms have also got ____ in them.

7. In an experiment, a living tissue is weighed (wet weight), dried (dry weight), and then fully burnt. The remaining material after complete burning is called ____ and it contains ____.

8. Inorganic compounds like sulphate, phosphate, etc., are also seen in the ____.

9. Therefore Elemental analysis gives the elemental composition of living tissues in the form of ____.

10. Analysis for compounds gives an idea of the ____ present in living tissues.

11. From a chemistry point of view, compounds are identified as ____ while from a biological point of view, they are classified into ____.

12. Amino acids are organic compounds containing an ____ and an ____ as substituents on the same carbon (α-carbon), hence they are called ____.

13. Amino acids are considered substituted methane because the central carbon has ____ occupying its four valency positions.

14. In amino acids, the four substituent groups attached to the α-carbon are hydrogen, amino group, carboxyl group, and ____.

15. Based on the nature of the R group, there are many ____.

16. However, the amino acids that occur in proteins are only of ____ types.

17. In proteinaceous amino acids, if the R group is hydrogen, methyl, and hydroxymethyl, the amino acids are called ____ respectively.

18. The chemical and physical properties of amino acids are essentially due to the ____.

19. Based on the number of amino and carboxyl groups, amino acids are classified as ____ and aromatic amino acids include ____.

20. A particular property of amino acids is the ____ nature of –NH2 and –COOH groups, hence their structure changes in solutions of different ____.

21. Lipids are generally ____.

22. Lipids could be simple ____.

23. A fatty acid has a ____ group attached to an R group.

24. In fatty acids, the R group could be ____.

25. For example, Palmitic acid has ____ carbon atoms including the carboxyl carbon.

26. Arachidonic acid has ____ carbon atoms including the carboxyl carbon.

27. Fatty acids are classified as saturated and unsaturated based on the presence or absence of ____.

28. Glycerol is a simple lipid which is chemically known as ____.

29. Many lipids are composed of both ____ and ____.

30. In many lipids, fatty acids are found ____ with glycerol.

31. Lipids formed by esterification of glycerol with fatty acids can be classified as ____.

32. Monoglycerides, diglycerides and triglycerides are also called fats and oils based on their ____.

33. Oils have ____ melting point and hence remain as oil/liquid (e.g., gingelly oil) even in winters.

34. Lipids that contain phosphorus and a phosphorylated organic compound are called ____ and they are found in ____.

35. Lecithin is an example of ____.

36. Some tissues, especially neural tissues, contain lipids with ____.

37. Living organisms have many carbon compounds in which ____ can be found.

38. Some heterocyclic compounds in living organisms are nitrogen bases such as ____.

39. When nitrogen bases are attached to a sugar, they are called ____.

40. If a phosphate group is also esterified to the sugar of a nucleoside, it is called a ____.

41. Which of the following are nucleosides?

42. Which of the following are nucleotides?

43. Nucleic acids like DNA and RNA consist of ____.

44. DNA and RNA function as ____.





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